cytometer Options
cytometer Options
Blog Article
Information-enriched characterization of mobile populations. Morphological facts from photos can insert towards the richness of flow cytometry knowledge. For instance, the determine reveals an in any other case typical apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Every inhabitants to reveal morphologically distinctive options. These insights couldn't are actually attained from multiplex staining on your own.
Whether or not you’re a newbie or an expert, take a look at our assets to advance your investigation and knowledge in flow cytometry.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The volume of singlets readily available for Evaluation raises once the consumer trains and employs a far more accurate product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a large-velocity brightfield digital camera for simultaneous high throughput flow cytometry and superior resolution brightfield imaging.
*Pricing is indicative only and is particularly subject to alter. You should Call your Agilent agent with any issues. The information established forth herein just isn't an offer or even a dedication, both equally of which can be issue to credit rating approval, stipulations, and availability with Agilent. Agilent Fiscal Methods is offered in picked international locations.
Moreover, it's illuminating to see how many doublets slip by means of conventional singlet gates, and terrific to verify the standing of unpredicted double-optimistic events in my analysis."
Pair your Attune flow cytometer by having an autosampler for greater workflow performance, and incorporate robotic integration for maximum throughput.
Constant graphic high-quality even at higher throughput Acoustic focusing and also a large-pace digital camera Merge to graphic these T cells constantly at minimal or higher flow fees. Quickly alter concentration and digital camera options to fulfill experimental necessities.
The Attune NxT cytometer is the trustworthy workhorse instrument your lab can depend upon for reliable and trustworthy efficiency. The Attune CytPix design features a high-pace brightfield digicam and AI-pushed picture Investigation software, with current upgrades including device Finding out applications and an intuitive person interface, enabling Improved knowledge precision, precision, and faster insights into sample biology.
Probably most of all, these activities contain cells of Evidently diverse phenotypes which could lead on to incorrect conclusions with regards to double positive functions (especially in uncommon populations).
Sound waves help exact check here mobile alignment, resulting in Fantastic knowledge good quality and ~10X more quickly sample flow costs.
Application Observe: Automated graphic analysis decreases consumer-to-person variability in flow cytometry gating methods
Designed-in dimensionality reduction instruments greater resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate anticipated populace resolution in lysed full human blood. Person can view activities from Just about every cluster for Visible affirmation of cell phenotypes.
No clean/no lyse leukocyte Evaluation with imaging. Cells were obtained from 24-hour-previous blood by dilution in one mM EDTA ( Skilled UX/UI enables consumers to coach AI versions for expanded applications. (A) The “Educate Model” feature supplies annotation instruments and serious-time product coaching precision.
Gating on these prolonged parameters permits you to quickly and precisely establish populations of curiosity and ensure gating strategy with little if any guide evaluation. The Attune computer software provides usage of state-of-the-artwork device Finding out Investigation instruments inside of an expertly created consumer interface, rendering it extremely easy to use.
The Attune Xenith cytometer is actually a large-parameter spectral analyzer, giving equally spectral unmixing and traditional flow capabilities for extensive and multipurpose mobile Examination.
The Attune CytPix Flow Cytometer will allow customers to visualize samples in the course of acquisition, confirm the precision of gating methods, and guidance effects with responsible documentation for publication reasons.
What gave the impression to be a homogeneous population is really far more assorted—an Perception that needs to be viewed as when interpreting outcomes.